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<t>Single-cell</t> <t>transcriptomic</t> analysis of liver fibrosis. (A) Quality control metrics before cell filtering, including the distribution of gene counts <t>(nFeature_RNA),</t> UMI counts (nCount_RNA), and the percentages of mitochondrial and hemoglobin genes across samples. (B) Cell clustering of liver fibrosis samples. (C) Cell-type annotation of single-cell <t>RNA-seq</t> data. (D) Cell cycle analysis of single-cell transcriptomic data. (E) Proportional changes of different cell types between normal and fibrotic groups. (F) Expression distribution of Acot9, Aldh1b1, and Pck2 across different cell types.
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Single-cell transcriptomic analysis of liver fibrosis. (A) Quality control metrics before cell filtering, including the distribution of gene counts (nFeature_RNA), UMI counts (nCount_RNA), and the percentages of mitochondrial and hemoglobin genes across samples. (B) Cell clustering of liver fibrosis samples. (C) Cell-type annotation of single-cell RNA-seq data. (D) Cell cycle analysis of single-cell transcriptomic data. (E) Proportional changes of different cell types between normal and fibrotic groups. (F) Expression distribution of Acot9, Aldh1b1, and Pck2 across different cell types.

Journal: Frontiers in Immunology

Article Title: Identification of mitochondria-related biomarkers in liver fibrosis via interpretable machine learning and WGCNA: transcriptomic analysis and In Vivo validation

doi: 10.3389/fimmu.2026.1705706

Figure Lengend Snippet: Single-cell transcriptomic analysis of liver fibrosis. (A) Quality control metrics before cell filtering, including the distribution of gene counts (nFeature_RNA), UMI counts (nCount_RNA), and the percentages of mitochondrial and hemoglobin genes across samples. (B) Cell clustering of liver fibrosis samples. (C) Cell-type annotation of single-cell RNA-seq data. (D) Cell cycle analysis of single-cell transcriptomic data. (E) Proportional changes of different cell types between normal and fibrotic groups. (F) Expression distribution of Acot9, Aldh1b1, and Pck2 across different cell types.

Article Snippet: Single-cell RNA sequencing (scRNA-seq) datasets were obtained from GSE145086 and GSE233084 , both generated using the 10X Genomics platform ( , ).

Techniques: Single Cell, Control, RNA Sequencing, Cell Cycle Assay, Expressing